A novel cDNA from Parthenium argentatum Gray enhances the rubber biosynthetic activity in vitro*

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A novel cDNA from Parthenium argentatum Gray enhances the rubber biosynthetic activity in vitro.

Natural rubber (cis-1,4-polyisoprene) is an isoprenoid compound produced exclusively in plants by the action of rubber transferase. Despite a keen interest in revealing the mechanisms of rubber chain elongation and chain length determination, the molecular nature of rubber transferase has not yet been identified. A recent report has revealed that a 24 kDa protein tightly associated with the sma...

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Natural rubber is an irreplaceable raw material vital to industry, transportation, medicine and defense. It is largely produced from clonal plantations of Hevea brasiliensis in southeastern Asia. Temperate-zone rubber-producing crops are greatly desired to increase biodiversity, protect supplies, and provide a safe natural-rubber alternative for the large numbers of people suffering from Type I...

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Magnesium ion regulation of in vitro rubber biosynthesis by Parthenium argentatum Gray.

Natural rubber is produced by a rubber transferase (a cis-prenyltransferase). Rubber transferase uses allylic pyrophosphate to initiate the rubber molecule and isopentenyl pyrophosphate (IPP) to form the polymer. Rubber biosynthesis also requires a divalent metal cation. Understanding how molecular weight is regulated is important because high molecular weight is required for high quality rubbe...

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Impairment of Photorespiratory Carbon Flow into Rubber by the Inhibition of the Glycolate Pathway in Guayule (Parthenium argentatum Gray).

Cut shoots of guayule (Parthenium argentatum Gray) were treated with four inhibitors of the glycolate pathway (alpha-hydroxypyridinemethanesulfonic acid; isonicotinic acid hydrazide, glycine hydroxamate, and amino-oxyacetate, AOA) in order to evaluate the role of photorespiratory intermediates in providing precursors for the biosynthesis of rubber. Photorespiratory CO(2) evolution in guayule le...

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Nucleotide sequence of a cDNA for a P2 60S acidic ribosomal protein from Parthenium argentatum.

In screening a stem-bark cDNA library from guayule (Parthenium argentatum) for genes involved in isoprenoid biogenesis, we isolated a full-length gene for a P2 60s acidic ribosomal protein that shows sequence similarity to vertebrate (Rich and Steitz, 1987), invertebrate (Qian et al., 1987), and yeast (Beltrame and Bianchi, 1987, 1990; Remacha et al., 1988) 605 acidic ribosomal proteins (Table ...

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ژورنال

عنوان ژورنال: Journal of Experimental Botany

سال: 2004

ISSN: 0022-0957,1460-2431

DOI: 10.1093/jxb/erh039